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. 2020 Jul 31;8:905. doi: 10.3389/fbioe.2020.00905

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Copyright © 2020 Sun, Lu, Liang, Yang and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytosine base editor-mediated gene inactivation in Pseudomonas putida KT2440. (A) Overview of the homogentisate pathway in P. putida KT2440. (B) Mutation alignment of the targeted HmgA spacer. (C) Phenotypes of hmgA knockout mutants. The hmgA mutant strains of P. putida KT2440 were streaked on selective plates. (D) The PobA spacer in pobA gene was selected for base editing to achieve gene inactivation. (E) Base editing results of Quic spacer in quiC gene. (F) Sequence alignment of the TtgA mutants after cytosine base editing.