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. 2020 Jul 31;8:905. doi: 10.3389/fbioe.2020.00905

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Copyright © 2020 Sun, Lu, Liang, Yang and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protocatechuic acid (PCA) production titer in KT2440::AroF2-P_J₂₃₁₁₉-derived strains after modification by the base editor. (A) A schematic representation of PCA synthesis in P. putida KT2440. (B) Genomic modification of KT2440::AroF2-P_J₂₃₁₁₉ by gene knockout and amino acid substitution using the base editor. (C) The PCA titer in different KT2440::AroF2-P_J₂₃₁₁₉-derived strains after shaking-flask fermentation in King’s medium. Abbreviations: DAHP, 3-deoxy-D-arabinoheptulosonate-7-phosphate. DHQ, 3-dehydroquinate. DHS, 3-dehydroshikimate. S3P, shikimate-3-phosphate. EPSP, 5-enolpyruvyl-shikimate-5-phosphate. PCA, protocatechuic acid.