FIGURE 2.
Expression of cMTO1 in CCl4-induced hepatic fibrotic tissues as well as primary HSCs. (A,B) Masson staining for assessing collagen deposition. Scale bar = 400 μm for 40×, 200 μm for 100× and 100 μm for 200×. The assessment of Masson area was finished at 100×. (C) Hydroxyproline level. (D) Col1A1 mRNA level. (E) circRNA splices derived from mouse MTO1 gene including mmu_circ_0015909, mmu_circ_0015910, mmu_circ_0015911, mmu_circ_0015912, mmu_circ_0015913, mmu_circ_0015914 and mmu_circ_0015915 were detected in CCl4 mice. (F) Expression of cMTO1 was analyzed in isolated primary HSCs at different culture time. *P < 0.05 compared to the control. Each value is the mean ± SD of three experiments.