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. 2020 Jul 31;8:714. doi: 10.3389/fcell.2020.00714

FIGURE 5.

FIGURE 5

The interaction between cMTO1 and miR-181b-5p. (A) The luciferase activity of pmirGLO-cMTO1-Wt in HEK-293T cells after co-transfection with the indicated 42 miRNAs or miR-NC. (B) A schematic drawing indicated the putative binding sites of miR-181b-5p with respect to cMTO1. Moreover, relative luciferase activities of pmirGLO-cMTO1-Wt or pmirGLO-cMTO1-Mut were analyzed in HEK-293T cells. (C) To validate the direct interaction between cMTO1 and miR-181b-5p, the pull-down assay was performed. Bio-miR-NC is not complementary to cMTO1. (D) Expression of miR-181b-5p in HSCs after Ad-cMTO1 or Ad-shcMTO1 treatment for 48 h. (E) Expression of cMTO1 in HSCs after miR-181b-5p transfection for 48 h. *P < 0.05. Each value is the mean ± SD of three experiments.