(A) TEM image of nanostructures formed after adding ALP (2 U/mL) to the solution of 1P-KMe3 (200 μM) for 24 h, in the presence of 0%, 5%, 10%, and 20% (w/v) PEG 20000. Scale bar, 100 nm.
(B) MD simulation snapshots of coarse-grained (CG) model with different volume fractions of crowders. Thick filament bundles are only observed in highly crowded environment.