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. 2020 Aug 6;10:13255. doi: 10.1038/s41598-020-69899-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemistry staining of SFRP1 in ampullary adenocarcinoma. (A) Negative expression of SFRP1. (B) High expression of SFRP1. (C) Kaplan–Meier analysis of the impact of SFRP1 expression on disease-specific survival in ampullary adenocarcinoma patients (P = 0.037). (D) Correlation between localisation of E-cadherin staining and expression of SFRP1 in using immunohistochemistry (IHC). High expression of SFRP1 is slightly correlated with the loss of E-cadherin, and low expression of SFRP1 is correlated with membranous staining of E-cadherin (P = 0.362). (E) Correlation between the localisation of β-catenin staining and expression of SFRP1 using IHC. High expression of SFRP1 is slightly associated with membranous staining of β-catenin, and low expression of SFRP1 is associated with nuclear staining of β-catenin (P = 0.301). IHC, immunohistochemistry; SFRP1, secreted frizzled related protein 1.