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Influence of the presence of varying amounts of 2-ME on the binding activity of HLA A*02:01. Various concentrations of A*02:01 were titrated for binding activity using radiolabeled HBV core 18–27 as a probe ligand, as described in Materials and Methods. Activity was assessed as a function of concentration of added 2-ME (A). Maximal counts of >85% could be obtained in the presence of 1 mM 2-ME (B).
