Table III.
Measurement of HLA-A*02:01 binding affinities of native and modified peptides in the presence or absence of 2-ME
| IC50 (ng/ml) |
||||||
|---|---|---|---|---|---|---|
| Ag | Sequence | Peptide Modification | Predicted | Normal | No Protease Inhibitors | 2-ME |
| CTAG2(157–165) | SLLMWITQC | Native | 390 | 13,487 | 820 | 157 |
| CTAG2(157–165) | SLLMWITQBa | P9 C to AABA | —b | 64 | 94 | 99 |
| HIVEP2(1674–1682) P1682L | CISSCNPNL | Native | 1,593 | >20,(KK) | >20,(KK) | 4,034 |
| HIVEP2(1674–1682) P1682L | BISSCNPNL | PI C to AABA | — | 212 | 573 | 250 |
| HIVEP2(1674–1682) P1682L | CISSBNPNL | P5 C to AABA | — | 845 | 1,047 | 618 |
| HIVEP2(1674–1682) P1682L | BISSBNPNL | PI, P5 C to AABA | — | 575 | 604 | 1,260 |
| HIVEP2(1674–1682) WT | CISSCNPNP | Native | >20,000 | >20,(KK) | >20,(KK) | >20,000 |
| HIVEP2(1674–1682) WT | BISSCNPNP | PI C to AABA | — | >20,(KK) | >20,(KK) | >20,000 |
| HIVEP2(1674–1682) WT | CISSBNPNP | P5 C to AABA | — | >20,(KK) | >20,(KK) | >20,000 |
| HIVEP2(1674–1682) WT | BISSBNPNP | PI, P5 C to AABA | — | 13,800 | 17,100 | 13,400 |
| BING4(alt orf) | CQWGRLWQL | Native | 20 | 1,425 | 1,132 | 155 |
| BING4(alt orf) | BQWGRLWQL | PI C to AABA | — | 143 | 121 | 93 |
| PHLPP 1(563–571) G556E | ILCETCLIV | Native | 60 | 461 | 325 | 306 |
| PHLPP 1(563–571) G556E | ILBETCLIV | P3 C to AABA | — | 63 | 58 | 82 |
| PHLPP 1(563–571) G556E | ILCETBLIV | P6 C to AABA | — | 162 | 104 | 93 |
| PHLPP 1(563–571) G556E | 1LBETBLIV | P3, P6 C to AABA | — | 18 | 14 | 24 |
| PHLPP 1(563–571) WT | ILCGTCLIV | Native | 110 | 192 | 544 | 228 |
| PHLPP 1(563–571) WT | ILBGTCLIV | P3 C to AABA | — | 419 | 516 | 808 |
| PHLPP 1(563–571) WT | ILCGTBLIV | P6 C to AABA | — | 1,395 | 839 | 885 |
| PHLPP 1(563–571) WT | ILBGTBLIV | P3, P6 C to AABA | — | 1,103 | 733 | 1,547 |
| CYFIP1016–124) 1121V | SCSRAVPQV | Native | 12,117 | 11,649 | 6,952 | 2,344 |
| CYFIP1016–124) 1121V | SBSRAVPQV | P2 C to AABA | — | 1,251 | 807 | 1,684 |
| CYFIP1016–124) WT | SCSRAIPQV | Native | 9,850 | 1,469 | 529 | 199 |
| CYFIP1016–124) WT | SBSRAIPQV | P2 C to AABA | — | 1,120 | 1,018 | 867 |
| CDK4(23–32) R24C | ACDPHSGHFV | Native | 18,383 | >20,(KK) | 3,302 | 13,477 |
| CDK4(23–32) R24C | ABDPHSGHFV | P2 C to AABA | — | 2,272 | 1,972 | 2,053 |
| CDK4(23–32) WT | ARDPHSGHFV | Native | >20,000 | >20,(KK) | >20,(KK) | >20,000 |
| GnTV(intron) | VLPDVFIRC | Native | 439 | 482 | 128 | 32 |
| GnTV(intron) | VLPDVFIRB | P9 C to AABA | — | 0.6 | 3.3 | 2.5 |
| TP53(168–176) R175H | HMTEVVRHC | Native | 4,984 | 4,210 | 1,850 | 1,440 |
| TP53(168–176) R175H | HMTEVVRHB | P9 C to AABA | — | 318 | 301 | 187 |
| TP53(168–176) WT | HMTEVVRRC | Native | 7,043 | 10,186 | 1,147 | 1,025 |
| TP53(168–176) WT | HMTEVVRRB | P9 C to AABA | — | 337 | 261 | 272 |
| PMEL(639–647) | RLPR1FCSC | Native | 1,492 | 1,975 | 512 | 68 |
| PMEL(639–647) | RLPR1FBSC | P7 C to AABA | — | 508 | 63 | 25 |
| PMEL(639–647) | RLPRIFCSB | P9 C to AABA | — | 11 | 11 | 27 |
| PMEL(639–647) | RLPRIFBSB | P7, P9 C to AABA | — | 4.7 | 7.2 | 14 |
a
B indicates AABA.
b
Dashes indicate that the binding affinities of these peptides could not be predicted as they contain amino acids that are not naturally incorporated into proteins.