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. 2020 Aug 6;10:13174. doi: 10.1038/s41598-020-68697-6

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Root tip somatic chromosome spread from African baobab A. digitata L. (Seedling-2). The total number of chromosomes is 168 (2n = 4x = 168), and they range from acrocentric to metacentric. Some of these chromosomes are shown in inserts (enlarged images of individual chromosomes) (M metacentric, NM near metacentric, SM sub-metacentric, AC acro-centric; the arrow heads point at the primary constrictions on individual chromosomes). Three massive Nucleolus Organizer Region (NOR) chromosomes were observed in Seedling-2 (see enlarged images marked as “NOR”). One smaller chromosome was masked by a larger one (see lower left-hand side boxes as shown in inserts, so the count should be two). Four chromosomes in oval encircled dotted line (at the top, center, are acro-centric chromosomes). To facilitate counting, the spread was divided into six sections of 27, 43, 42, 13, 27 and 16 chromosomes, respectively, for a total of 2n = 4x = 168. Scale bar is 5 µm.