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. 2020 Aug 6;10:13174. doi: 10.1038/s41598-020-68697-6

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

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A portion of a metaphase chromosome spread of Adansonia digitata L. (Seedling-2), with all three massive 45S rDNA-bearing chromosomes and the fourth chromosome shown in inset (top-left hand side corner). (a) An FITC (fluorescein isothiocyanate) image (green signal, curly bracket) of 45S rDNA was captured with automatic camera mode (to obtain a very bright green image). (b) The image of same spread as in “a” was captured with bright DAPI and reduced FITC in controlled camera mode to display bright chromosome background with low green (45S rDNA) signal. (c) Same spread as in “a” with 45S rDNA and ATRS probe signals. (d) Same spread as in “a”, image was captured under reduced FITC that displays faint green string of green signal (curly brackets) where the ATRS signals are brightly visible at the end of the respective NORs. (e) Same spread as in “a” without green signal. (f) Same spread as in “a” with only bright DAPI-stained chromosomes. The right-hand side Figure is a diagrammatic representation of the four 45S rDNA chromosomes of Seedling-2. Scale bar is 5 µm.