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. 2020 Aug 6;10:13174. doi: 10.1038/s41598-020-68697-6

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Root tip chromosome spreads of Adansonia digitata L. (Seedling-1), analyzed with 45S rDNA and ATRS-type telomere oligonucleotide probes. (a) Four 45S rDNA (green) and telomere repeat (red) signals in DAPI-stained chromosomes background, inserts are the enlarged images of 45S rDNA-bearing chromosomes (with no green signals) showing the telomere signals at both ends of each chromosome, arrow heads show the telomere signals at the terminal end of each 45S rDNA signals. (b) Same cell as in “a” captured with short exposure time under FITC filter, the telomere signals at the terminal end of each 45S rDNA signals (light green signals) are brightly visible compared to “a”. Scale bar is 5 µm.