Table 2.
List of oligonucleotide probes used for fluorescent in situ hybridization*a.
| No | Probe | Sequence | Length | Modification |
|---|---|---|---|---|
| 1 | 18S_UniOP_1 | CCGGAGAGGGAGCCTGAGAAACGGCTAC | 28 | 5′AlexaFluor488 |
| 2 | 18SrDNA_UniOP_2 | ATCCAAGGAAGGCAGCAGGCGCGCAA | 26 | 5′AlexaFluor488 |
| 3 | 18SrDNA_UniOP_3 | GGGCAAGTCTGGTGCCAGCAGCCGCGGT | 28 | 5′AlexaFluor488 |
| 4 | 18SrDNA_UniOP_4 | TCGAAGACGATYAGATACCGTCSTAGT | 27 | 5′AlexaFluor488 |
| 6 | 5.8SrDNA_UniOP_1 | AAYGACTCTCGGCAACGGATATCTMG | 26 | 5′AlexaFluor488 |
| 7 | 5.8SrDNA_UniOP_2 | CWYGCATCGATGAAGAACGTAGCRA | 25 | 5′AlexaFluor488 |
| 8 | 5.8SrDNA_UniOP_3 | GCGATACTTGGTGTGAATTGCAGAATC | 27 | 5′AlexaFluor488 |
| 9 | 5.8SrDNA_UniOP_4 | GTGAACCATCGAGTYTTTGAACGCAAGT | 28 | 5′AlexaFluor488 |
| 5 | 5SrDNA_ang_1 | GGATGCGATCATACCAGCACTAAAGCACCG | 30 | 5′Cy3 |
| 6 | 5SrDNA_ang_2 | CCCATCAGAACTCCGAAGTTAAGCGTGCT | 29 | 5′Cy3 |
| 7 | 5SrDNA_ang_3 | GCGAGAGTAGTACTAGGATGGGTG | 24 | 5′Cy3 |
| 8*b | 5SrDNA | TCAGAACTCCGAAGTTAAGCGTGCTTGGGCGAGAGTAGTAC | 41 | 5′Cy3 |
| 9 | Telomere | TTTAGGGTTTAGGGTTTAGGGTTTAGGGT | 29 | 5′TexRd-X, 5′Cy3 |