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. 2020 Aug 6;10:13234. doi: 10.1038/s41598-020-70204-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Receiver operating characteristic (ROC) curve analysis. Antigen-specific (antigen-nil) release of IFN-γ in the tested in-house tubes. Areas under the curve (AUCs) from each tested tube were comparable: in-house QFN AUC = 0.9858 (green line), in-house QFN + A AUC = 0.938 (orange line), in-house QFN + B AUC = 0.9761 (blue line) and in-house QFN + C AUC = 0.9748 (purple line). Active tuberculosis patients and latently infected individuals with a QFN-G-IT positive result were considered as positive controls and QFN-G-IT negative healthy controls as negative controls.