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. 2020 Aug 6;11:3904. doi: 10.1038/s41467-020-17667-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a A panel of NIP30 truncations co-expressed with flag-REGγ in 293T cells was analyzed for interaction domains by immunoprecipitation. b A summary of the interaction between REGγ and various NIP30 derivative clones (+ for binding, − for no binding). c Multiple sequence alignment showing the evolutionarily conserved, serine-rich REGγ-interacting motif in NIP30. d NIP30 is phosphorylated at 4 specific serine residues. HA-tagged NIP30 with phosphorylation-mimetic (S/D) or defective (S/A) mutations were tested for interaction with REGγ by immunoprecipitation. e Generation of NIP30 expressing cells. H1299 cells with or without REGγ (shN/shR) stably expressing NIP30 constructs (WT, 4A, or 4D) were examined for p21 expression. f H1299 cells expressing distinct NIP30 constructs were analyzed for cell proliferation by MTT assays. Data represent mean ± SEM (n = 4). g Xenograft tumors derived from H1299 cells stably expressing different NIP30 constructs (WT/4A/4D) were compared for tumor size. Quantitative results are in Supplementary Fig. 3n. Data in this figure are representative of three independent repeats. Source data are provided in a Source Data file.