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. 2020 Aug 6;11:3904. doi: 10.1038/s41467-020-17667-7

Fig. 4. The CDC25A–NIP30-REGγ pathway regulates p21 during the cell cycle.

Fig. 4

a, REGγ WT and 293T KO cells were synchronized to the G0/G1, S, and G2/M phases. Cell lysates were subjected to western blot analysis with indicated antibodies. Data are representative of at least three independent experiments. b Synchronized REGγ WT and KO 293T cells were treated with NSC95397 for 3 h. Cell lysates were subjected to western blot analysis. Data represent mean ± SEM from three independent experiments. c REGγ WT and KO 293T cells were transfected with an siRNA specific for CDC25A or flag-CDC25A for 72 h. Cells synchronized in the S phase were collected for western blot analysis. Data represent mean ± SEM from three independent experiments. d 293T cells were transfected with a short interfering RNA (-si) for NIP30 or an si control for 72 h. Cells arrested at the S phase were collected for western blot analysis in three independent experiments. Data represent mean ± SEM. e REGγ WT and KO 293T cells were transfected with indicated short interfering RNA (-si) for NIP30 or NIP30 4A/4D constructs. Cells synchronized at the S phase were collected for western blots in three independent experiments. Data represent mean ± SEM. Source data are provided in a Source Data file.