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. 2020 Aug 6;11:3904. doi: 10.1038/s41467-020-17667-7

Fig. 6. NIP30 mimetics enhance chemotherapeutic effects in p53-deficient cells.

Fig. 6

a Summary of alterations for NIP30 in different cancer types from the TCGA project. The alteration types include mutation (green), amplification (red), deep deletion (blue) and multiple alternations (gray). b NIP30 phosphorylation mimetics enhance anticancer drug efficiency. H1299 cells stably overexpressing WT, 4A, or 4D NIP30 were treated with indicated concentrations of etoposide, cisplatin, 5-FU, doxorubicin for 48 h followed by MTT assays (n = 4). c Xenograft tumors were generated by injecting H1299, and H1299 overexpressing NIP30 4A/4D cells into dorsal flanking sites of nude mice. Two weeks later, mice were treated with cisplatin (5 mg/kg), or doxorubicin (3 mg/kg), or 5-Fluorouracil (20 mg/kg), three times per week i.p. for 2 weeks. All experiments were repeated three times. d Quantitation of the results in panel c. Values are presented as the means ± SEM. *P < 0.05, ***P < 0.001, (one-way ANOVA). P (lane 1, lane 3) = 0.024, P (lane 1, lane 4) = 1.8E-8, P (lane 1, lane 7) = 3.9E-8, P (lane 1, lane 10) = 3.6E-4, P (lane 4, lane 6) = 0.03, P (lane 7, lane 9) = 0.027, P (lane 10, lane 12) = 1.5E-5. e A model depicting the CDC25A–NIP30-REGγ pathway in regulation of the cell cycle and DNA damage response. Source data are provided in a Source Data file.