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. 2020 Aug 6;10:13211. doi: 10.1038/s41598-020-70043-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Visualization of the signal quantification method. The signal in 20 periodically chosen slices of the myocardium was normalized to a 1% agarose reference probe and the pre-contrast agent scan. The reference probe is highlighted with a red arrow. (B) After injection of LIBS-MPIO a signal decrease was observed. The signal differed significantly from control-MPIO injected mice 20 and 40 min after injection. Animal numbers LIBS-MPIO: n = 12, control-MPIO: n = 9. (C) Visualization of the signal decrease in LIBS-MPIO injected mice (upper row) and control-MPIO injected mice (lower row). Blue indicates a lower signal in the myocardium compared to the pre contrast agent scan. (D) Histological counting of MPIOs in the myocardium showed significantly higher numbers of LIBS-MPIOs compared to control-MPIOs. (E) Platelet count was similar in both groups.