Figure 2.

Phenotypic analyses of multiple PAP2-encoding genes knockout strains. (A) DOC sensitivity assay: 5 µl of serial dilutions of the indicated cells were laid down on 2YT-agar plates supplemented (down) or not (up) with 20 mg/ml of DOC. Approximate numbers of bacteria (CFU), according to the A_600nm of a liquid culture, which are present in the original drops, are indicated on the right. (B) Silver stained SDS–polyacrylamide gel showing migration patterns of LPS isolated from the different BW25113 WT and deletion mutant strains. (C) Fitness cost of simultaneous PAP2 and PBP inactivation. BW25113, BWΔmrcA::Cam^R (PBP1A inactivated), BWΔmrcB::Cam^R (PBP1B inactivated), DMEG9 (PgpB, YbjG and LpxT inactivated), BW[BacA][PBP1A] (PgpB, YbjG, LpxT and PBP1B inactivated) were grown in 2YT at 37 °C. Experiments were performed in triplicate, and the data shown are from independent experiments.