Table 2.
In trans complementation of the thermosensitive strains.
| Plasmida | Protein | BWTsbacA | BWTetra-TsbacA | BWPGPTs | |||
|---|---|---|---|---|---|---|---|
| − IPTG | + IPTGb | − IPTG | + IPTG | − IPTG | + IPTG | ||
| None | − | − | − | − | − | − | |
| pTrcHis60 | None | − | − | − | − | − | − |
| pTrcBac30 | BacA | + | NT | + | NT | − | − |
| pTrcH60ybjG | YbjG | + | NT | + | NT | − | − |
| pTrcH60lpxT | LpxT | − | − | − | − | − | − |
| pTrcH60pgpB | PgpB | + | + | + | + | + | + |
| pTrcH30ynbD | YnbD | − | − | − | − | − | − |
| pTrcH60pgpA | PgpA | − | − | − | − | + | + |
| pTrcH60pgpC | PgpC | − | − | − | − | + | + |
| C1motif | |||||||
| pA727 | K97A | + | NT | + | NT | + | |
| pA730 | ΔV100 | − | + | − | + | + | NT |
| pA733 | R104A | − | + | − | + | + | NT |
| pA737 | P105A | + | NT | + | NT | + | NT |
| C2motif | |||||||
| pB877 | E154A | + | NT | + | NT | + | NT |
| pA741 | A158S | + | NT | + | NT | + | NT |
| pA745 | F159A | + | NT | + | NT | + | NT |
| pA762 | P160A | + | NT | + | NT | + | NT |
| pA766 | S161A | − | + | − | + | + | NT |
| pA771 | G162A | + | + | − | + | + | NT |
| pA749 | G162D | − | − | − | − | − | − |
| pA798 | H163A | + | NT | + | NT | + | NT |
| pB881 | E154A/H163A | + | + | − | + | − | + |
| pA802 | T164A | + | NT | + | NT | + | NT |
| C3motif | |||||||
| pA807 | S200A | + | NT | + | NT | + | NT |
| pA812 | R201A | − | + | − | + | + | NT |
| pA817 | L202A | + | NT | + | NT | + | NT |
| pA822 | G205A | + | NT | + | NT | + | NT |
| pA828 | H207A | − | − | − | − | − | − |
| pA833 | W208A | + | NT | + | NT | + | NT |
| pA838 | P209A | + | NT | + | NT | + | NT |
| pA843 | D211A | − | + | − | + | + | NT |
| pA847 | L212A | + | NT | + | NT | + | NT |
| pA852 | A215G | + | NT | + | NT | + | NT |
| pA857 | S219A | + | NT | + | NT | + | NT |
aThe plasmids carrying a copy of the different lipid phosphatase encoding genes or pgpB variants were tested for their ability to restore the growth at 42 °C of the three thermosensitive strains. + , normal growth at 42 °C; −, no growth at 42 °C; NT, not tested.
bThe growth at 42 °C was also monitored in the presence of IPTG in the growth medium at 1 mM in all cases, except for lpxT overexpression (10 µM). In bold boxes are indicated the variants displaying a complete or partial defect of complementation.