Table 2. Summary of the impact of chimerisation on TbAQP2 localisation and function.
|
Protein |
Sequence source |
Localisation |
Half-life (h) |
EC50 pentamidine (nM)1 |
EC50 SHAM (μM)1 |
EC50 SHAM + 5 mM glycerol (μM)1 |
|---|---|---|---|---|---|---|
| TbAQP1 | Wild type | Flagellar base | 4.34 | 10.08 | 3.02 | 1.45 |
| TbAQP2 | Wild type | Flagellar pocket | 4.83 | 3.29 | 1.96 | 1.16 |
| TbAQP3 | Wild type | Plasma membrane | 1.15 | 10.23 | 1.28 | 1.14 |
| 40AT | Chimera | Plasma membrane | 1.64 | 10.34 | 1.39 | 1.12 |
| AQP2TMD4 | Chimera | Plasma membrane | <1h | 10.24 | 0.74 | 0.64 |
1Estimated EC50 values from cells induced with tetracycline, 1μg/ml for 24h.