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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Nat Cell Biol. 2020 Apr 27;22(5):559–569. doi: 10.1038/s41556-020-0492-1

Extended Data Figure 3. D2868K mutation has no effect on GST-dyneinMOTOR motility.

Extended Data Figure 3.

(A) Schematic of the plasmid used to produce GST-dyneinMOTOR (wild-type and D2868K mutant). Restriction digest with ApaI (cuts within URA3 gene) targets the plasmid for homologous recombination into the ura3–1 locus as depicted. (B) Cartoon representation of the minimal GST-dimerized dynein motor domain (amino acids 1219–4092 of the dynein heavy chain, Dyn1). (C and D) Plots depicting mean values (left) and all values (right) for velocity (C) and run length (D) of wild-type and D2868K GST-dyneinMOTOR, along with the standard error (n = 217 and 238 motors for each).