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. 2020 Jul 21;6(4):e484. doi: 10.1212/NXG.0000000000000484

Figure 2. Confocal microscopy of DM1 myoblasts.

Figure 2

MBNL1 protein (green), RNA foci (red), nucleus (blue). Scale bars: 2.5 µm. (A) XY and XZ volume slices of individual channels corresponding to nucleus (blue), MBNL1 protein (green), and RNA foci (red). DM1 myoblast showing the nuclear colocalization between the RNA foci and the MBNL1 protein. (B) Merged image of XY and XZ volume slices of representative nuclear colocalization between RNA foci and MBNL1 protein in DM1 myoblast. White arrow indicates a single RNA foci aggregate colocalizing with MBNL1 protein. (C) Isosurface rendering of nuclear colocalization between RNA foci and MBNL1 protein in DM1 myoblasts. (D) XY and XZ volume slices of individual channels corresponding to nucleus (blue), MBNL1 protein (green), and RNA foci (red). DM1 myoblast showing the cytoplasmic colocalization between the RNA foci and the MBNL1 protein. (E) Merged image of XY and XZ volume slices of representative cytoplasmic colocalization between RNA foci and MBNL1 protein in DM1 myoblast. White arrow indicates a single RNA foci aggregate colocalizing with MBNL1 protein. (F) Isosurface rendering of cytoplasmic colocalization between RNA foci and MBNL1 protein in DM1 myoblasts. MBNL1 = muscleblind like 1.