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. 2020 Aug 3;13:7629–7639. doi: 10.2147/OTT.S253137

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© 2020 Liu et al.

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PPAT was highly expressed in TC tissues and cells. (A) We used IHC to detect the expression of PPAT protein in tissue samples from 50 TC patients and 50 normal thyroid tissues. (B) qRT-PCR was used to detect the expression of PPAT mRNA in TC tissues and normal thyroid tissues. (C) qRT-PCR was used to detect PPAT mRNA expression in normal thyroid follicular epithelial cells and TC cells. (D) Western blot was used to detect PPAT protein expression in normal thyroid follicular epithelial cells and TC cells. (E) The relationship between the expression of PPAT and the prognosis of TC patients was analyzed by GEPIA database. The experimental results were analyzed by Student’s t-test, and the difference was statistically significant with P < 0.05. **P < 0.01 and ***P < 0.001.