Fig. 3. Strategic addition of signaling antagonists to modulate OPC specification.

(A) Timing of addition for three inhibitory signaling cues—GANTT61, IWP-2, and DAPT—in the OPC differentiation protocol. (B) i. Olig2⁺, Nkx2.2⁺, and the proportion of total Olig2⁺ that are Nkx2.2⁺/Olig2⁺ cells in at day 21 in response to full factorial combinations of selected novel signaling antagonists. ii. Immunocytochemistry images of costained Olig2 (red) and Nkx2.2 (green) cells. Scale bar, 100 μm. Error bars represent 95% confidence intervals from four technical replicates.