Localization of DNAH8 and Associated Protein DNAH17 in the Spermatozoa from Men Harboring Bi-allelic DNAH8 Variants
(A) Immunofluorescence staining of the spermatozoa from a normal control male and subjects carrying bi-allelic DNAH8 variants. Anti-DNAH8 (red) and anti-α-tubulin (green) antibodies were used. Spermatozoa were counterstained with 4′,6-diamidino-2-phenylindole as a marker of the cell nucleus. In the fertile control male, DNAH8 immunostaining (red) concentrated along the sperm flagella, but this signal was almost absent from the sperm flagella from subjects harboring bi-allelic DNAH8 variants. The data of subject A051 are provided to illustrate the typical staining observed in the DNAH8-associated cases. Scale bars: 5 μm.
(B) DNAH17 immunostaining is affected in the spermatozoa from men harboring bi-allelic DNAH8 variants. The spermatozoa were stained with anti-DNAH17 (red) and anti-α-tubulin (green) antibodies. DNAH17 staining mainly localized along the spermflagella from a control male, while being evidently reduced in the spermatozoa from subject A051. Scale bars: 5 μm.