Pups Obtained upon ICSI using the Spermatozoa from Dnah8-KO Male Mice
(A) Development of ICSI embryos. Fourteen (17.3%) of the 81 oocytes injected with the spermatozoa from Dnah8-KO (Dnah8em1/em1) male mice developed to the two-cell stage, and three pups were obtained after embryo transfer. ICSI data from the use of sperm from wild-type male mice are shown as controls.
(B) The pups obtained from ICSI using the spermatozoa from Dnah8-KO male mice.
(C) Genotyping of these pups. All of the pups carried the heterozygous Dnah8-mutated allele. KO denotes the mutated allele and WT denotes the wild-type allele.