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. 2020 Jul 18;104(17):7367–7376. doi: 10.1007/s00253-020-10700-1

Table 1.

Primer sequences used in this study

Oligo nucleotide Sequence (5′ to 3′)
lcp1_F CATATGGAACCAATGAGCAGGCG
lcp1_R CTCGAGGGTCGGGCGGTTGGTG
lcp2_F CATATGAAACGCAGAGTTCTGCTGTCC
lcp2_R AAGCTTTTCGGGCCTGTTGATGG
lcp3_F CATATGCAAAATCTCAGCAGACG
lcp3_R GAGCGCTGACAAGCTTGGCG
lcp1_lcp2_F2 CGACATCATGGTCACCTGGCACAG
lcp1_lcp2_R2 CCCTTGGAGTAGTAGACCGACCAG
lcp2_oxiB_F2 GATCATCAGCCAGGAGGACATTCTC
lcp2_oxiB_R2 GTCGAGGTCCATCGCGAAGGTCTTG
oxiB_oxiA_F2 CAGTTCAGCCATACATCGCCGTACC
oxiB_oxiA_R2 TTGAGATGACTGGTGCACGCCC
QRTlcp1_F GCGGACCTGGAAGAAGAAC
QRTlcp1_R TAGAGGACGCCGAGGTAGAG
QRTlcp1_Probe CCGTCGACTTCAACGAGAAG (FAM-TAMRA)
QRTlcp2_F GAGGCCTGGTCGGTCTACTA
QRTlcp2_R CCAGGTGACGAGAATGTCCT
QRTlcp2_Probe GTGACTGCGTCGTCAACG (FAM-TAMRA)
QRTlcp3_F CGTACGGCTACGACCTCAGT
QRTlcp3_R GTTGCTGATCGGGATGAAGT
QRTlcp3_Probe CGTCACCTCGAACAAGACG (FAM-TAMRA)
QRT16sOR16_F AGCGTTGTCCGGATTTATTG
QRT16sOR16_R CCTCCTGATATCTGCGCATT
QRT16sOR16_Probe AGGCTAGAGTTCGGTAGGGG (FAM-TAMRA)
tetR_lcp1_F GGCGCCGAGTGAATCCATCG
tetR_lcp1_R CGATGTAAGGAGACCGGCAT
lcp3_UP_F CAGCGGAGCGAGCTGCATGC
lcp3_UP_R CATTCGCTTACCTACTTACG