Fig. 3.

ETV6–RET and EGFR–SEPT14 translocations. Archer FusionPlex Custom Solid Panel demonstrates ETV6–RET translocation (ETV6 breakpoint, exon 6; RET breakpoint, exon 12) and EGFR–SEPT14 translocation (EGFR breakpoint, exon 24; SEPT14 breakpoint, exon 7) (a). RT-PCR amplification with 2 sets of primers verifies EGFR–SEPT14 fusion by both gel electrophoresis (b) [M, DNA ladder; Lane 1, amplified 237 bp fusion gene; Lane 2, HAPMAP Normal RNA control; Lane 3, water] and Sanger sequencing (c)