Table 4.

Detail of the primers of 12 randomly selected DEGs for qRT-PCR

Gene no.	Gene ID	Description	Primers sequence (5′–3′)	Production (bp)	log2Foldchange (RNA-Seq)	log2Foldchange (qRT-PCR)
1	D-TRINITY_ DN33510_c1_g1_i1	ribulose-1,5-bisphosphate carboxylase small subunit	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	177	7.68	6.5
2	D-TRINITY_ DN47859_c3_g5_i1	Unknown	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	169	6.39	5.4
3	D-TRINITY_ DN24736_c1_g1_i1	chitinase family protein	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	167	6.1	5.9
4	D-TRINITY_ DN47873_c3_g7_i6	Unknown	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	158	4.9	3.7
5	D-TRINITY_ DN43139_c2_g1_i13	Rab GTPase activator	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	146	3.2	2.7
6	D-TRINITY_ DN68230_c0_g1_i1	NAC transcription factor	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	166	3.03	1.8
7	D-TRINITY_ DN32373_c1_g2_i1	Unknown	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	150	− 6.15	− 4.6
8	D-TRINITY_ DN38456_c0_g1_i3	myb transcription factor	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	148	− 5.34	− 5.4
9	D-TRINITY_ DN32684_c0_g1_i1	hitinase family protein	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	175	− 5	− 4.6
10	D-TRINITY_ DN37999_c1_g1_i6	ethylene response factor	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	165	− 3	− 1.9
11	D-TRINITY_ DN46507_c0_g1_i2	auxilin-related protein	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	151	− 3.9	− 4.3
12	D-TRINITY_ DN24815_c0_g1_i1	Unknown	Forward:CTTTGAGTTGAAGCATGAAGCA Reverse:ATTGTTGGCCTTCTTGGTAACCG	148	− 2.9	− 2