Fig. 3.

Carotenoid structures identified in R. marinus strain ISCaR-493 (A), mutant TK-1 (ΔtrpBΔpurAΔcrtYO::trpB) (B) and mutant TK-3 (ΔtrpBΔpurAΔcruFcrtB::trpBcrtB_{T.thermophilus}) (C). The top row shows chemical formulas of identified carotenoids, corresponding theoretical masses and detected masses by MS. The middle row shows absorbance spectra from 350 to 600 ​nm of carotenoids isolated with acetone:hexane (1:1) and A_max values of obtained peaks. Graphs with both axes are shown in Figs. S17-S19. The bottom row shows the chemical structures of identified carotenoids: Native carotenoids (4-keto 2‘-hydroxy β,ψ-carotene acyl glycoside), modified lycopene (2’-hydroxy ψ,ψ-carotene acyl glycoside) and lycopene. For more information on R₁ and R₂, refer to Fig. 1.