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. 2020 Aug 6;10:13297. doi: 10.1038/s41598-020-69990-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MtDod-PAS-Pep purity and stability. (a) SEC profile of FMN:mtDod-PAS-Pep1. The dodecameric species can be identified by the FMN absorption at 375 nm and 450 nm. In addition, unbound FMN is visible at ~ 117 mL. All constructs, except mtDod-PAS-Pep3, showed comparable chromatographic profiles. (b) SDS-PAGE gel of all purified mtDod-PAS-Pep constructs. Use of standard loading buffer (pH 6.8, 2.5% SDS) at prolonged heat incubation (30 min, 95 °C) allows the observation of monomer and dodecamer. (c) Thermocyclic fluorescence assay of mtDod-PAS-Pep constructs. Measurements were performed in PBS and at pH 4.7 as described above (see Fig. 4).