Table 2.
MtDod constructs for AB production. After purification by the heat treatment protocol, the correct size of the proteins was verified by ESI–MS.
| mtDod constructs | Peptide sequence | Calculated mass without start-Met/Da | Measured mass by MS (+ 1 H+)/Da |
|---|---|---|---|
| mtDod-PAS-Pep1 | PKGGSGSGPTIEEVD | 10,155 | 10,156.7 |
| mtDod-PAS-Pep2 | PLEGDDDTSRMEEVD | 10,434 | 10,435.0 |
| mtDod-PAS-Pep3 | ECYPNEKNSVNMDLD | 10,497 | 10,803.4* |
| mtDod-PAS-Pep4 | VPSDSDKKLPEMDID | 10,415 | 10,416.1 |
| mtDod-PAS-Pep5 | DSSQHTKSSGEMEVD | 10,363 | 10,363.9 |
| mtDod-PAS-Pep6 | EQSTGQKRPLKNDEL | 10,469 | 10,470.2 |
| mtDod-PAS-Pep7** | ALMVYRCAPPRSSQF | 10,453 | – |
| mtDod-PAS-Pep8 | LVTGESLEQLRRGLA | 10,368 | 10,369.1 |
| mtDod-PAS-Pep9 | MKGKEEKEGGARLGA | 10,287 | 10,288.0 |
| mtDod-PAS-Pep10 | EERRIHQESE | 10,038 | 10,039.6 |
| mtDod-PAS-Pep11 | NHEGDEDDSH | 9,880 | 9,881.3 |
| mtDod-PAS-H7 | HHHHHHH | 9,704 | 9,705.2 |