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. 2020 Aug 6;10:13296. doi: 10.1038/s41598-020-69921-z

Figure 2.

Figure 2

Performance of Human Chagas-Flow ATE-IgG1 for universal serodiagnosis of Chagas disease. (A) Two-graph receiver operating characteristic (TG-ROC) analysis of performance indices [Sensitivity (Se) and Specificity (Sp)] along a range of cut-offs for Human Chagas-Flow ATE-IgG1 applied to universal diagnosis of Chagas disease using distinct target antigens. The gray background underscored the range of cut-off values (Δ = min and max ranges) with identical Se and Sp for each target antigen. TG-ROC curve profiles pre-selected the pairs of attributes “AI 250”, “EVI 250”, “AII 250”, “TII 250” and “EII 250” as those with broader cut-off ranges to segregated sera samples from Chagas disease patients (CH) from non-infected controls (NI). (B) Representative scatter plot distributions of IgG1 reactivity at individual level further illustrate the ability of the pre-selected set of attributes to discriminate the IgG1 reactivity observed for CH (black dots) from NI (gray dots). The results are expressed as the percentage of positive fluorescent parasites (PPFP) with the dotted line representing the cut-offs defined by the TG-ROC analysis. Color backgrounds (red for TcI, green for TcVI and blue for TcII) highlight the sets of attributes (“target-antigen/serum dilution/cut-off”) with the higher performance for the universal diagnosis of Chagas disease, including “AI 250/40”, “EVI 250/30%”, “AII 250/40%”, “TII 250/40% and “EII 250/30%”.