Table 1.

Observed end-to-end DNA error rates, which includes errors introduced during synthesis, sample handling and storage, preparation, and sequencing

		Mutagenesis kit			50°C incubation
	Untreated	Low	Medium	High	2°h	8°h
Substitution	0.0057	0.0075	0.0178	0.0238	0.0082	0.0085
Deletion	0.0054	0.0045	0.0067	0.0082	0.0040	0.0047
Insertion	0.0023	0.0020	0.0032	0.0039	0.0017	0.0019
Total	0.0134	0.0139	0.0277	0.0359	0.0140	0.0151

The observed total error rates on the order of 1% were approximately doubled and tripled by the medium and high protocols (respectively) of the mutagenesis kit. Incubation at 50 °C for 2 and 8 h had only a small effect and was not further tested.