Fig. 1.
In vitro characterization of Fc-modified variants of VRC07-523-LS. The indicated mutations were introduced on the backbone of the VRC07-523LS antibody and tested for the following. (A) Neutralization of SHIVSF162P3 and a six-virus HIV panel (values are IC80, µg/mL). (B) Binding to rhesus macaque Fcγ receptors by biolayer interferometry. KD values are shown within each graph. (C) ADCC against CEM cells chronically infected with SHIVSF162P3. Data are representative of at least three experiments using NK cells isolated from peripheral blood of naïve monkeys. (D) Phagocytosis of beads coated with RSC3 by THP-1 cells. The y axis shows phagocytosis score calculated as percentage of cells bound by beads multiplied with the MFI of beads. Data are representative of at least three experiments. (E) Binding to C1q and C3c from rhesus macaque serum, n = 3. Data are mean ± SD and representative of at least two independent experiments.