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. 2020 Jul 20;117(31):18530–18539. doi: 10.1073/pnas.2008923117

Fig. 3.

Fig. 3.

Colocalization of Vps8 with Vps13 decreases in response to rapamycin treatment. (A) Fluorescence images of cells expressing Vps13-GFP 0 and 3 h after rapamycin treatment. The vacuolar membrane was stained with FM4-64. (B) Fluorescence images of cells expressing Vps13-GFP and Vps8-6xmCherry at the indicated time points in response to rapamycin treatment. The arrowheads point to Vps13 puncta colocalized with Vps8 puncta. (C) Quantification of Vps8-6xmCherry puncta that colocalize with Vps13-GFP puncta for cells in B. Error bars represent SEM; N = 3 independent experiments. **P < 0.01, Student’s t test. (D) Quantification of Vps13-GFP puncta that colocalize with Vps8-6xmCherry in cells shown in B. Error bars represent SEM; n = 3 independent experiments. NS, nonsignificant (P ≥ 0.05), Student’s t test. (E) Fluorescence images of yeast cells expressing Vps8-6xmCherry at the indicated time points in response to rapamycin treatment. (F) Quantification of Vps8-6xmCherry puncta that localize to the vacuolar membrane for cells in E. Error bars represent SEM; N = 3 independent experiments. NS, nonsignificant (P ≥ 0.05), Student’s t test. (Scale bars, 2 μm.) DIC, differential interference contrast.