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. 2020 Jul 20;117(31):18530–18539. doi: 10.1073/pnas.2008923117

Fig. 5.

Fig. 5.

ER, but not mitochondria, accumulates at the late endosome in the vps13∆ mutant. (A) Rtn1 accumulates at the late endosome in the vps13∆ mutant. Fluorescence images of cells expressing Rtn1-GFP and Vps8-6xmCherry at the indicated time points in response to rapamycin treatment. The arrowheads mark Vps8 puncta that colocalize with Rtn1. (B) The percentage of Vps8 puncta colocalizing with Rtn1 was quantified. Error bars represent SEM; N = 3 independent experiments. NS, nonsignificant (P ≥ 0.05), **P < 0.01, Student’s t test. (C) Mitochondria do not accumulate at the late endosome in the vps13∆ mutant. Fluorescence images of cells expressing Cox4-GFP and Vps8-6xmCherry at the indicated time points in response to rapamycin treatment. The arrowheads mark Vps8 puncta that colocalize with Cox4. (D) The percentage of Vps8 puncta colocalizing with Cox4 was quantified. Error bars represent SEM; N = 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Student’s t test. (Scale bars, 2 μm.)