Fig. 5.

KRM1-dependent uncoating of CV-A10 in vitro. (A–D) Representative negative stain EM images of four CV-A10 virus groups without (A and B) or with (C and D) KRM1 proteins and exposed to neutral pH (pH 7.4, Upper Left) or acidic pH (pH 5.5) treatments. Samples were negatively stained immediately after mixing or after a 10-min incubation at 37 °C. (E) The percentage of empty particles for each group is displayed in the interleaved scatterplot, with a horizontal line indicating the median. The significance of differences was tested by Tukey’s multiple comparison test. ****P < 0.0001; ns, not significant.