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. 2020 Jul 20;117(31):18701–18710. doi: 10.1073/pnas.2005726117

Fig. 4.

Fig. 4.

YY1 binds to the YY1 binding site in the HTLV-1 R region DNA in vitro, but the binding site is not required for transactivation in vivo. (A) Electrophoretic mobility shift assays with recombinant His-tagged YY1 were performed using biotin-labeled HTLV-1 R YY1-binding site (WT-YY1 probe), or Scrambled (ScramYY1 probe), or deleted (DelYY1 probe) YY1-binding site DNAs. Anti-YY1 (lane 2) or nonspecific IgG (lane 3) antibodies were used for supershift assay. Unmodified probes (WT-YY1 or Del-YY1) were used for competition experiments. Data shown are representative of two independent experiments. (B) Similar experiments in A using HTLV-1 R fragments (Upper). Location of YY1 binding site (YY1 BS) is indicated by the "*". AAV-P5 denotes adeno-associated virus P5 promoter DNA. MLV-PBS denotes Moloney murine leukemia virus primer-binding site DNA. Data shown are representative of two independent experiments. (C) Similar experiments in Fig. 1B using HTLV-1 LTR YY1-binding site 4 deletion mutant. Results shown are means ± SEs from three independent experiments performed in duplicate.