Fig. 6.

YY1 binds to HTLV-1 R in vitro and in vivo. (A) Bacterially expressed His-tagged YY1 was subjected to an in vitro RNA-binding assay using biotin-labeled HTLV-1 R RNA or control RNA. (Upper) In vitro synthesized RNA used for pulldown was visualized using agarose electrophoresis. (Lower) Formation of YY1–RNA complex monitored using WB with an anti-His antibody. (B) UV-crosslink RNA immunoprecipitation (RIP) of HEK-293 cells transfected with HA-YY1 together with HTLV-1 R luciferase followed by qRT-PCR with primers specific for HTLV-1 R or tubulin. RIP data are presented as percent of input RNA (means ± SEs from three independent experiments). Student’s t test was used for statistical analysis. *P < 0.05.