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. 2020 Jul 20;117(31):18431–18438. doi: 10.1073/pnas.2006893117

Fig. 1.

Fig. 1.

Design and characterization of the P7-based FP probe. (A) Schematic of the FP assay to identify molecules with affinity to the HA stem. The polarization signal of the P7-carboxytetramethylrhodamine (TAMRA) probe produces high and low FPs when bound or unbound to the HA protein, respectively. (B) Chemical structure of the P7-TAMRA probe. (C) P7-TAMRA probe (75-nM) and H1/PR8 (30-nM) competition assay against a dose-dependent increase in P7 peptide or stem-targeting bnAb S9-3–37 millipolarization (mP). (D) P7-TAMRA probe (75 nM) and other HA (A/California/04/2009 [H1/Cal04] [30-nM] or A/Michigan/45/2015 [H1/Mich15] [100-nM]) competition assays against the P7 peptide.