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. 2020 Jul 20;117(31):18431–18438. doi: 10.1073/pnas.2006893117

Fig. 2.

Fig. 2.

HTS for H1/PR8 and hit confirmation. (A) Scatter plot representation of 15 384-well plates in HTS with F0045 shown in blue. Black dots represent the number of compounds screened and red dots represent a peptide P7 control (coefficient of variation [CV]). (B) Chemical structure of the best hit F0045 from the HTS campaign. (C) Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the TS assay of 5-μM H1/PR8 HA incubated with 50-μM P7, P7-TAMRA probe, or F0045 with DMSO as a negative control. F0045 protects against trypsin degradation at pH 5.0 in comparison to the DMSO control (*).