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. 2020 Jul 16;117(31):18661–18669. doi: 10.1073/pnas.2007667117

Fig. 5.

Fig. 5.

Htt134Q:EGFP ubiquitination affects the cellular proteome. Proteomic profile of soluble fractions from HEK293 cells expressing FU-Htt17Q:EGFP-Wm, FU-Htt134Q:EGFP-Wm, or FU-Htt134Qm:EGFP-Wm extracted with 10% SDS (Gel, three replicates from one experiment) or NP-40 (six replicates from two experiments). Both populations were analyzed by MS as described in Materials and Methods. (A) Numbers of proteins up-regulated in Htt134Q:EGFP- relative to Htt134Qm:EGFP-expressing cells and vice versa. (B) Biological process enrichment of the differentially expressed proteins shown in A analyzed using the STRING database version 11.0. (i) Data derived from analysis of gel-resolved proteins; (ii) data derived from NP-40 dissolved proteins.