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. 2020 Aug 7;11:3984. doi: 10.1038/s41467-020-17848-4

Fig. 2. IP3R1 SD and epsin 1 UIM domains interact under atherogenic conditions.

Fig. 2

a to c Full length and domain-deletion constructs of FLAG-epsin 1 in the pcDNA3 vector were transfected into HEK 293T (293tsA1609neo) cells for 24 h, followed by IP and western blot analysis using antibodies against HA (IP3R1) or FLAG (epsin 1) tags, as well as specific proteins (n = 5). d–f Full length and deletion constructs of HA-IP3R1 were transfected into HEK 293T cells for 24 h, followed by IP and western blot analysis using antibodies against HA or FLAG tags and specific proteins (n = 5). g to i Fine mapping of the N-terminus of IP3R1 to determine the region that interacts with epsin 1. Interaction results are presented as a 0–100 scale mapping domain(s) that bind(s) epsin 1 upon co-expression in 293T cells (n = 6, asterisk P < 0.001, compared to the fragments deleted SD). Transfected HEK 293T cells were all treated with 100 μg/mL oxLDL for 30 min. NTD N-terminal domain, RD regulatory domain, TMD transmembrane domain, CTT C-terminal cytoplasmic tail, SD suppressor domain, IBC IP3 binding core composed of α and β domains.