Skip to main content
. 2020 Aug 7;10:13343. doi: 10.1038/s41598-020-70231-7

Figure 1.

Figure 1

IL-17A is constitutively expressed by some human TNBC cell lines and further upregulated upon IL-23 and LPS stimulation. (A) IL-17A mRNA expression levels in 53 different human breast cancer cell lines, including 19 TNBC cell lines, were extracted from C-bioportal. (B/C) Human triple-negative breast cancer cell line HS578T was cultured in medium alone or stimulated with human IL-23 (10 ng/mL) plus LPS (1 µg/mL) for various times points. Total RNAs were extracted at 6, 18, and 24 h post-treatment, and IL-17A mRNA expression was examined by RT-PCR. The data is expressed as the mean ± SEM of 6–8 replicates per time point. The statistical analysis was conducted using one-way ANOVA with Dunnett’s multiple comparisons test. ***P < 0.001 comparing to untreated control (B). Whole-cell extracts were collected at 24 and 48 h post-treatment, and IL-17A protein was detected by western blot where β-actin was used as a loading control. The relative levels of IL-17A were determined using scanning densitometry (C).