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. 2020 Aug 7;10:13343. doi: 10.1038/s41598-020-70231-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AdIL-17A transduction in 4T1 cells induces expansion of both Ly6G^hi and Ly6G^int granulocytic populations. Peripheral blood samples were collected from naïve or tumor-bearing mice at days 4, 7, and 14 post tumor inoculation and stained for FACS analysis (n = 10 per group). The analyses were conducted by first gating on CD45⁺ cells. (A) Representative dot plots of subpopulations of Gr1⁺/CD11b⁺ cells in naïve, AdIL-17A, and Addl groups. (B,C) Frequency (out of CD45⁺cells) and the absolute number of granulocytic (G: CD11b⁺Ly6C^intLy6G^hi) cells (D,E) Frequency and the absolute number of monocytic (M: CD11b⁺Ly6C^hiLy6G^lo) cells. (F–I) The absolute number of Arg-1- and PD-L1-expressing granulocytic and monocytic GR1⁺/CD11b⁺ cells in AdIL-17A and Addl groups at days 7 and 14 post-tumor inoculation. *P < 0.05; ***P < 0.001 compared to Addl, ^##P < 0.01; ^###P < 0.001 compared to D0, using two-way ANOVA with Dunnett’s multiple comparisons test.