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. 2020 Aug 7;10:13326. doi: 10.1038/s41598-020-70242-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of MS on the adolescent offspring anxiety assessed in the EPM. (A) time in open arms (%); (B) number of open arms entries; (C) time in closed arms (%); (D) time in centre area (%); (E) time immobile (%); (F) total entries; (G) number of rearing; (H) number of head dipping. Data were analysed by two-way ANOVA (2 × 2 factorial design) with Bonferroni comparisons except for: (B) Data were analysed by logistic regression. (A), (C), (D), (E) data were arcsine transformed. (F), (G), (H) data were square-root transformed. n = 44 except for Kyoto control (n = 36). Results are expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.