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. 2020 Aug 7;11:3978. doi: 10.1038/s41467-020-17818-w

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Fig. 8 — a Knockdown of individual SAA enzymes in HepG2 cells has distinct impacts on expression of epithelial and mesenchymal markers. Indicated HepG2 cells were analyzed for expression of epithelial and mesenchymal markers by qPCR (n = 4 replicates per group). b Supplementation of metabolites in the transsulfuration pathway partially represses the expression of HNF4α depletion-induced mesenchymal markers in HepG2 cells. SiNeg or siHNF4α HepG2 cells were cultured in the complete medium with or without 1 mM Ctt, 1 mM NaSH, or 10 mM taurine and cultured for 48 h. The relative expression levels of the indicated epithelial and mesenchymal markers were analyzed by qPCR (n = 4 replicates per group). c Overexpression of CBS partially inhibits cell migration induced by HNF4α depletion. SiNeg and siHNF4α HepG2 cells were transfected with either an empty vector (pcDNA3) or a pcDNA3-CBS construct expressing CBS. Cells were then analyzed for cell migration in a transwell assay (n = 5 replicates per group). The number of migrated control cells (siNeg + pcDNA3) was normalized to 1. Bar, 500 μm. d Overexpression of SAA metabolic genes or HNF4α represses migration of HNF4α-negative SNU449 cells. SNU449 cells were transfected with either an empty vector (pcDNA3) or a pcDNA3 construct expressing the indicated SAA enzyme. Cells were then analyzed for cell migration in a transwell assay (n = 5 replicates per group). The number of migrated control cells (pcDNA3) was normalized to 1. Bar, 500 μm. e Overexpression of individual SAA enzyme in HNF4α-negative SNU449 cells has distinct impacts on expression of the expression of epithelial and mesenchymal markers. Control (pcDNA3) SNU449 cells, and SNU449 cells overexpressing individual SAA enzyme were analyzed for expression of epithelial and mesenchymal markers by qPCR (n = 4 replicates per group). For all bar graphs, values are expressed as mean ± s.e.m., two-tailed, unpaired Student’s t-test, *p < 0.05.