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. 2020 Aug 7;11:3981. doi: 10.1038/s41467-020-17718-z

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© The Author(s) 2020, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–c Three independent replicate experiments. Western blotting of TG in 293T cells that were either untransfected (a, no detectable bands) or transfected with constructs encoding mouse TG wild type (WT) or P118L or G67S point mutants (in the pcDNA3.1 background, in which the CMV promoter drives the respective cDNA expression). Serum-free media (M) were collected overnight and the cells (C) were lysed. Equal volumes of media and cells were analyzed by SDS-PAGE, electrotransfer to nitrocellulose, and immunoblotting with anti-Tg-specific antibodies. Full scans of western blotting are presented in Supplementary Fig. 14. From scanning densitometry, d shows the content of thyroglobulin (and its variants) intracellularly and in the secretion. e The extracellular : intracellular (M/C) ratio of each construct. d, e Three independent replicate experiments. All boxplots in d and e indicate median (center line), 25th and 75th percentiles (bounds of box), and minimum and maximum (whiskers).