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. 2020 Jul 16;117(31):18617–18626. doi: 10.1073/pnas.1919379117

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Fig. 2. — Tbx3 maintains pacemaker and conduction properties in the AV node. (A) Approach to obtain and define transcriptomes of WT and Tbx3^+/− AV nodes. (B) Volcano plot of normalized expression levels in WT AV nodes over Tbx3^+/− AV nodes. All significantly up-regulated genes are labeled green, and all significantly down-regulated genes are labeled red. P < 0.05 (red and green), P < 0.1 (light red and light green). (C) Average WT expression levels (in TPM) of conduction system and chamber genes. (D) Unsupervised hierarchical clustering of genes differentially expressed between Tbx3^+/− AV nodes and WT AV nodes. GO terms and KEGG pathways associated with up-regulated (green) and down-regulated (red) genes in Tbx3^+/− AV nodes. (E) Representative immunostaining for Hcn4 (red), CoxIV (green), and nuclei (blue). Graph shows quantification of CoxIV signal intensity in AVN normalized to WM in WT and Tbx3^+/− sections (n = 3). AVN, AV node; WM, working myocardium. (Scale bar: 10 µm.)